Ever since monoclonal antibodies were produced in 1975 with mouse myeloma cells there has been interest in developing human myeloma cultures for the production of monoclonal antibodies.However, despite multiple attempts, no human myeloma line suitable for hybridoma production has been described.This way of producing hybrid molecules is superior to the production of hybrid antibodies by chemical reconstitution methods because the drastic treatment required for chain separation in the latter is likely to lead to some protein denaturation and loss of antibody activity.
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The availability of this cell line should enable the The technique for the production of monoclonal antibodies was a culmination of a range of experimental studies with murine myeloma cell lines (1, 2).
However, the mouse myelomas were not suitable for deriving human monoclonal antibodies because the heterospecific hybrids quickly lost the relevant human chromosomes.
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